Journal: Breast Cancer : Targets and Therapy

Article Title: The Correlation Between CD8+ Tumor-Infiltrating Lymphocytes and the Efficacy of Neoadjuvant Therapy in Breast Cancer

doi: 10.2147/BCTT.S533799

Figure Lengend Snippet: Immunohistochemistry slides of CD8+ TILs. ( A ) Baseline CD8+ TILs under ×200 magnification. Black arrows indicate iCD8+ TILs, and red arrows indicate sCD8+ TILs. Both intratumoral and stromal CD8+ TILs are diffusely distributed, with sCD8+ TILs being more abundant than iCD8+ TILs. ( B ) Baseline CD8+ TILs under ×400 magnification, showing positive staining of the cell membrane and cytoplasm (indicated by arrows).

Article Snippet: The CD8 rabbit anti-human polyclonal antibodies and secondary antibodies were sourced from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., and the immunohistochemistry staining kit was procured from Fuzhou Maixin Biotechnology Development Co., Ltd. Formalin-fixed, paraffin-embedded tissue sections were prepared according to standard procedures, including sectioning, deparaffinization, and rehydration.

Techniques: Immunohistochemistry, Staining, Membrane

Journal: Cell Reports Medicine

Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density

doi: 10.1016/j.xcrm.2025.102378

Figure Lengend Snippet: hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and CD8-positive cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .

Article Snippet: Anti-human CD8 , CST , Cat#85336.

Techniques: Activity Assay, Cell Culture, Immunohistochemistry, Staining, Immunohistochemical staining, Immunofluorescence